Simone
Once does you make mistakes,you don't have another chance.
Once you fail/make a mistake, there will be no second chance.
fail - 失败
make a mistake - 犯错误
Once you make a mistake you have no second opportunity（或者chance）.
Once you have made a mistake, there would be no second chance.
once you have failed，you will not have second chance
Once you mistake, there will be no second chance
